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primary bronchial epithelial cell culture primary bronchial  (ATCC)


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    ATCC primary bronchial epithelial cell culture primary bronchial
    Primary Bronchial Epithelial Cell Culture Primary Bronchial, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary bronchial epithelial cell culture primary bronchial/product/ATCC
    Average 99 stars, based on 518 article reviews
    primary bronchial epithelial cell culture primary bronchial - by Bioz Stars, 2026-02
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    The M4 compound is a broadly-acting influenza virus inhibitor: ( A ) Chemical structure of M4. (B) After pretreatment with M4 for 16h at the indicated concentrations, A549 cells were infected with influenza A/WSN/33 virus at MOI of 0.1, (C) MEF cells were infected with at MOI of 0.5 and (D) HTBE cells were infected at MOI of 0.25. 48h post infection, cells were fixed, stained for NP, and analyzed with a high content immunofluorescence imager. Percent infection was calculated as the ratio of anti-NP-stained cells to DAPI stained cells. Data are normalized by the mean for DMSO-treated wells and are shown as means ± SEM from three independent experiments. Dose-response curves for infectivity (black) and cell number (red) are shown. (E) MDCK cells were pre-treated for 16hs with different concentrations of M4 followed by infection with the indicated influenza A subtypes at MOI of 0.1 or influenza B/Yamagata at MOI of 0.2. Supernatants were analyzed at 24h post infection by plaque assay and data are represented as means ± s.d. of three independent experiments. The IC 50 values are indicated in .

    Journal: bioRxiv

    Article Title: Small molecule inhibition of the mitochondrial lipid transfer protein STARD7 attenuates influenza viral replication

    doi: 10.64898/2026.01.20.700505

    Figure Lengend Snippet: The M4 compound is a broadly-acting influenza virus inhibitor: ( A ) Chemical structure of M4. (B) After pretreatment with M4 for 16h at the indicated concentrations, A549 cells were infected with influenza A/WSN/33 virus at MOI of 0.1, (C) MEF cells were infected with at MOI of 0.5 and (D) HTBE cells were infected at MOI of 0.25. 48h post infection, cells were fixed, stained for NP, and analyzed with a high content immunofluorescence imager. Percent infection was calculated as the ratio of anti-NP-stained cells to DAPI stained cells. Data are normalized by the mean for DMSO-treated wells and are shown as means ± SEM from three independent experiments. Dose-response curves for infectivity (black) and cell number (red) are shown. (E) MDCK cells were pre-treated for 16hs with different concentrations of M4 followed by infection with the indicated influenza A subtypes at MOI of 0.1 or influenza B/Yamagata at MOI of 0.2. Supernatants were analyzed at 24h post infection by plaque assay and data are represented as means ± s.d. of three independent experiments. The IC 50 values are indicated in .

    Article Snippet: HTBE cells (ATCC PCS-300-010) were cultured in commercially available airway epithelial cell basal media following the manufacturer’s protocol (ATCC).

    Techniques: Virus, Infection, Staining, Immunofluorescence, Plaque Assay